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Welcome to the Human Pain Genetics Lab!


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Luda Diatchenko, MD, Ph.D

Canada Excellence Research Chair in Human Pain Genetics

Persistent pain is part of many common human clinical conditions, yet the current ability to diagnose and manage these conditions is inadequate. Pain perception is one of the most complicated measurable traits, as it is an aggregate of other measurable phenotypes associated with peripheral and central nervous system dynamics, stress responsiveness, and inflammatory state. It is generally accepted that complex traits, like pain perception, result from the interplay between environmental exposures and multiple genetic variants. However, little is known about the nature of these genetic variants. Due to the established role of environmental exposures, and the common view that pain perception is an unquantifiable and subjective experience, a genetic basis for pain perception has long been questioned. Thanks to recent and rapidly developing discoveries in the field of pain genetics, there is now evidence for genetic background playing a substantial role in pain perception and clinical pain phenotypes. These findings provide unique opportunities to identify genetic variants that contribute to pain phenotypes. The development of genome-wide approaches assures global and unbiased assessment of these genetic contributions.

The Human Pain Genetics lab investigates the psychological, molecular, cellular, and genetic pathways that mediate both acute and persistent pain states. Our primary goal is to identify the critical elements of human genetic variability contributing to pain sensitivity and pathophysiological pain states that will enable individualized treatments and therapies. Other related research endeavors include molecular hierarchy and evolution of functional SNPs (single-nucleotide polymorphisms), regulation of gene expression underlying molecular pain signaling, testing of surrogate pain animal models for genes influencing human pain, and clustering of neurological and psychological phenotypes that contribute to human persistent pain conditions. Answering these questions requires collaboration with experts in both clinical and basic biological sciences. These collaborative activities allow us to take basic genetic findings all the way from human association studies, through molecular and cellular mechanisms, to animal models, and ultimately to human clinical trials.

Current research projects at the Human Pain Genetics lab include:

  • Identifying critical elements of human genetic variability contributing to pain sensitivity and pathophysiological pain states through genome-wide approaches.
  • Identifying SNP-dependent regulation of gene function underlying variability in human molecular pain signaling.
  • Identifying ways to enable individualized treatments and therapies for human pain.
Acute and Persistent Pain States

Organization Logos
Our Team

People


The Human Pain Genetics Lab comprises a diverse group of scientists, not only with a wide range of areas of expertise such as molecular biology, neuroscience, dentistry, genetics, medicine, and bioinformatics, but also with different levels of training, from undergraduate students completing their honors projects to postdoctoral fellows and academic faculty. Cultural diversity is also embraced as one of our great assets, with members joining us from many parts of the world, creating a stimulating and exciting environment for professional growth. Former trainees from our lab have also gone on to develop successful careers in a variety of fields, both in academia and the private sector.

Name Position More Information
Luda Diatchenko, MD, PhD Principal Investigator
Phone: 514-398-2878
E-mail: luda.diatchenko@mcgill.ca
CV Bio
Photograph of Luda Diatchenko, MD, PhD
Samar Khoury, PhD Research Associate
Phone: 514-398-2833
E-mail: samar.khoury2@mcgill.ca
Photograph of Samar Khoury, PhD
Ryan Lichtenwalter, PhD Data Analyst
Phone: 514-398-3176
E-mail: ryan.lichtenwalter@mcgill.ca
CV Bio
Photograph of Ryan Lichtenwalter, PhD
Marc Parisien, PhD Research Associate
Phone: 514-398-2717
E-mail: marc.parisien@mcgill.ca
CV Bio
Photograph of Marc Parisien, PhD
Vivek Verma, BDS, MSc PhD Candidate
Phone: 514-398-2717
E-mail: vivek.verma@mail.mcgill.ca
CV
Photograph of Vivek Verma, BDS, MSc
Katerina Zorina-Lichtenwalter, MA, PhD Post-doctoral Researcher
Phone: 514-398-2833
E-mail: katerina.lichtenwalter@mail.mcgill.ca
CV
Photograph of Katerina Zorina-Lichtenwalter, MA, PhD
Francesca Montagna, MA Research Assistant
Phone: 514-398-8366
E-mail: francesca.montagna@mcgill.ca
Photograph of Francesca Montagna, MA
Xiang Ao, PhD Post-doctoral Researcher
Phone: 514-398-2717
E-mail: xiang.ao@mcgill.ca
Photograph of Xiang Ao, PhD
Kelly Cool, BA Clinical Research Coordinator
Phone: 514-398-5971
E-mail: kelly.cool@mail.mcgill.ca
Photograph of Kelly Cool, BA
Adam Trefonides Systems Administrator
Phone: 514-398-3176
E-mail: adam.trefonides@mcgill.ca
Photograph of Adam Trefonides
Name Position More Information
Diversity Map

Past Members

Name Position More Information
Xia (Shawn) Wen Undergraduate
Photograph of Xia (Shawn) Wen
Meijuan Niu Lab Manager
Photograph of Meijuan Niu
Hao Huang Lab Manager
Photograph of Hao Huang
Yerkebulan Talzhanov Post-doctorate
Photograph of Yerkebulan Talzhanov
Lisanne Plein Master's Candidate
Photograph of Lisanne Plein
Julia Segal Undergraduate
Photograph of Julia Segal
Natalie Zacchia, MPH Clinical Research Administrator
Photograph of Natalie Zacchia, MPH
Alexander Samoshkin, PhD Faculty Lecturer
Photograph of Alexander Samoshkin, PhD
Stefano Cattaneo, MD, BSc, MSc Master's Graduate
Photograph of Stefano Cattaneo, MD, BSc, MSc
Rodrigo Benavides, MD Post-doctorate
Photograph of Rodrigo Benavides, MD
Maria Verner Undergraduate
Photograph of Maria Verner
Richard Klares Undergraduate
Photograph of Richard Klares
Maria Di Nezza Administrative Assistant
Photograph of Maria Di Nezza
Sarah Jane Martinez, MSc Chief Research Technician
Photograph of Sarah Jane Martinez, MSc
Anne-Julie Chabot-Doré, PhD Post-doctorate
Photograph of Anne-Julie Chabot-Doré, PhD
Pavel Gris, MD, PhD Senior Research Associate
Lab Manager
Photograph of Pavel Gris, MD, PhD
Nicol Tugarinov Undergraduate
Photograph of Nicol Tugarinov
Marjo Piltonen, PhD Research Associate
Photograph of Marjo Piltonen, PhD
Sonali Uttam, MSc Research Assistant
Photograph of Sonali Uttam, MSc
Carol Meloto, DDS, PhD Post-doctorate
Photograph of Carol Meloto, DDS, PhD
Name Position More Information
Publications

Publications


Peer-Reviewed Publications
Stabilization of μ-opioid receptor facilitates its cellular translocation and signaling. Zhu, Han, Samoshkin, Convertino, Linton, Faison, Ji, Diatchenko, Dokholyan. Proteins, 87(10). 2019.
Premorbid and concurrent predictors of TMD onset and persistence. Ohrbach, Slade, Bair, Rathnayaka, Diatchenko, Greenspan, Maixner, Fillingim. European journal of pain (London, England). 2019.
Cartilage-binding antibodies induce pain through immune complex-mediated activation of neurons. Bersellini Farinotti, Wigerblad, Nascimento, Bas, Morado Urbina, Nandakumar, Sandor, Xu, Abdelmoaty, Hunt, Ängeby Möller, Baharpoor, Sinclair, Jardemark, Lanner, Khmaladze, Borm, Zhang, Wermeling, Cragg, Lengqvist, Chabot-Doré, Diatchenko, Belfer, Collin, Kultima, Heyman, Jimenez-Andrade, Codeluppi, Holmdahl, Svensson. The Journal of experimental medicine, 216(8). 2019.
A functional substitution in the L-aromatic amino acid decarboxylase enzyme worsens somatic symptoms via a serotonergic pathway. Khoury, Piltonen, Ton, Cole, Samoshkin, Smith, Belfer, Slade, Fillingim, Greenspan, Ohrbach, Maixner, Neely, Serohijos, Diatchenko. Annals of neurology, 86(2). 2019.
Clinical predictors of persistent temporomandibular disorder in people with first-onset temporomandibular disorder: A prospective case-control study. Meloto, Slade, Lichtenwalter, Bair, Rathnayaka, Diatchenko, Greenspan, Maixner, Fillingim, Ohrbach. Journal of the American Dental Association (1939), 150(7). 2019.
A study in scarlet: MC1R as the main predictor of red hair and exemplar of the flip-flop effect. Zorina-Lichtenwalter, Lichtenwalter, Zaykin, Parisien, Gravel, Bortsov, Diatchenko. Human molecular genetics, 28(12). 2019.
Profound analgesia is associated with a truncated peptide resulting from tissue specific alternative splicing of DRG CA8-204 regulated by an exon-level cis-eQTL. Upadhyay, Zhuang, Diatchenko, Parisien, Kang, Sarantopoulos, Martin, Smith, Maixner, Levitt. PLoS genetics, 15(6). 2019.
Alternative Splicing of the Delta-Opioid Receptor Gene Suggests Existence of New Functional Isoforms. Piltonen, Parisien, Grégoire, Chabot-Doré, Jafarnejad, Bérubé, Djambazian, Sladek, Geneau, Willett, Stone, Shabalina, Diatchenko. Molecular neurobiology, 56(4). 2019.
Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain. Parisien, Samoshkin, Tansley, Piltonen, Martin, El-Hachem, Dagostino, Allegri, Mogil, Khoutorsky, Diatchenko. Pain, 160(4). 2019.
CACNG2 polymorphisms associate with chronic pain after mastectomy. Bortsov, Devor, Kaunisto, Kalso, Brufsky, Kehlet, Aasvang, Bittner, Diatchenko, Belfer. Pain, 160(3). 2019.
Genome-wide association reveals contribution of MRAS to painful temporomandibular disorder in males. Smith, Parisien, Bair, Belfer, Chabot-Doré, Gris, Khoury, Tansley, Torosyan, Zaykin, Bernhardt, de Oliveira Serrano, Gracely, Jain, Järvelin, Kaste, Kerr, Kocher, Lähdesmäki, Laniado, Laurie, Laurie, Männikkö, Meloto, Nackley, Nelson, Pesonen, Ribeiro-Dasilva, Rizzatti-Barbosa, Sanders, Schwahn, Sipilä, Sofer, Teumer, Mogil, Fillingim, Greenspan, Ohrbach, Slade, Maixner, Diatchenko. Pain, 160(3). 2019.
Disentangling the genetics of lean mass. Karasik, Zillikens, Hsu, Aghdassi, Akesson, Amin, Barroso, Bennett, Bertram, Bochud, Borecki, Broer, Buchman, Byberg, Campbell, Campos-Obando, Cauley, Cawthon, Chambers, Chen, Cho, Choi, Chou, Cummings, de Groot, De Jager, Demuth, Diatchenko, Econs, Eiriksdottir, Enneman, Eriksson, Eriksson, Estrada, Evans, Feitosa, Fu, Gieger, Grallert, Gudnason, Lenore, Hayward, Hofman, Homuth, Huffman, Husted, Illig, Ingelsson, Ittermann, Jansson, Johnson, Biffar, Jordan, Jula, Karlsson, Khaw, Kilpeläinen, Klopp, Kloth, Koller, Kooner, Kraus, Kritchevsky, Kutalik, Kuulasmaa, Kuusisto, Laakso, Lahti, Lang, Langdahl, Lerch, Lewis, Lill, Lind, Lindgren, Liu, Livshits, Ljunggren, Loos, Lorentzon, Luan, Luben, Malkin, McGuigan, Medina-Gomez, Meitinger, Melhus, Mellström, Michaëlsson, Mitchell, Morris, Mosekilde, Nethander, Newman, O'Connell, Oostra, Orwoll, Palotie, Peacock, Perola, Peters, Prince, Psaty, Räikkönen, Ralston, Ripatti, Rivadeneira, Robbins, Rotter, Rudan, Salomaa, Satterfield, Schipf, Shin, Smith, Smith, Soranzo, Spector, Stancáková, Stefansson, Steinhagen-Thiessen, Stolk, Streeten, Styrkarsdottir, Swart, Thompson, Thomson, Thorleifsson, Thorsteinsdottir, Tikkanen, Tranah, Uitterlinden, van Duijn, van Schoor, Vandenput, Vollenweider, Völzke, Wactawski-Wende, Walker, J Wareham, Waterworth, Weedon, Wichmann, Widen, Williams, Wilson, Wright, Yerges-Armstrong, Yu, Zhang, Zhao, Zhou, Nielson, Harris, Demissie, Kiel, Ohlsson. The American journal of clinical nutrition, 109(2). 2019.
Anatomical selectivity in overlap of chronic facial and bodily pain. Slade, Rosen, Ohrbach, Greenspan, Fillingim, Parisien, Khoury, Diatchenko, Maixner, Bair. Pain reports, 4(3). 2019.
Low back pain. Vlaeyen, Maher, Wiech, Van Zundert, Meloto, Diatchenko, Battié, Goossens, Koes, Linton. Nature reviews. Disease primers, 4(1). 2018.
The human pain genetics database: an interview with Luda Diatchenko. Diatchenko. Pain management, 8(4). 2018.
Human carbonic anhydrase-8 AAV8 gene therapy inhibits nerve growth factor signaling producing prolonged analgesia and anti-hyperalgesia in mice. Zhuang, Upadhyay, Tong, Kang, Erasso, Fu, Sarantopoulos, Martin, Wiltshire, Diatchenko, Smith, Maixner, Levitt. Gene therapy, 25(4). 2018.
Human pain genetics database: a resource dedicated to human pain genetics research. Meloto, Benavides, Lichtenwalter, Wen, Tugarinov, Zorina-Lichtenwalter, Chabot-Doré, Piltonen, Cattaneo, Verma, Klares, Khoury, Parisien, Diatchenko. Pain, 159(4). 2018.
Genetic studies of human neuropathic pain conditions: a review. Zorina-Lichtenwalter, Parisien, Diatchenko. Pain, 159(3). 2018.
The more you test, the more you find: The smallest P-values become increasingly enriched with real findings as more tests are conducted. Vsevolozhskaya, Kuo, Ruiz, Diatchenko, Zaykin. Genetic epidemiology, 41(8). 2017.
Erratum: Large meta-analysis of genome-wide association studies identifies five loci for lean body mass. Zillikens, Demissie, Hsu, Yerges-Armstrong, Chou, Stolk, Livshits, Broer, Johnson, Koller, Kutalik, Luan, Malkin, Ried, Smith, Thorleifsson, Vandenput, Hua Zhao, Zhang, Aghdassi, Åkesson, Amin, Baier, Barroso, Bennett, Bertram, Biffar, Bochud, Boehnke, Borecki, Buchman, Byberg, Campbell, Campos Obanda, Cauley, Cawthon, Cederberg, Chen, Cho, Jin Choi, Claussnitzer, Collins, Cummings, De Jager, Demuth, Dhonukshe-Rutten, Diatchenko, Eiriksdottir, Enneman, Erdos, Eriksson, Eriksson, Estrada, Evans, Feitosa, Fu, Garcia, Gieger, Girke, Glazer, Grallert, Grewal, Han, Hanson, Hayward, Hofman, Hoffman, Homuth, Hsueh, Hubal, Hubbard, Huffman, Husted, Illig, Ingelsson, Ittermann, Jansson, Jordan, Jula, Karlsson, Khaw, Kilpeläinen, Klopp, Kloth, Koistinen, Kraus, Kritchevsky, Kuulasmaa, Kuusisto, Laakso, Lahti, Lang, Langdahl, Launer, Lee, Lerch, Lewis, Lind, Lindgren, Liu, Liu, Liu, Ljunggren, Lorentzon, Luben, Maixner, McGuigan, Medina-Gomez, Meitinger, Melhus, Mellström, Melov, Michaëlsson, Mitchell, Morris, Mosekilde, Newman, Nielson, O'Connell, Oostra, Orwoll, Palotie, Parker, Peacock, Perola, Peters, Polasek, Prince, Räikkönen, Ralston, Ripatti, Robbins, Rotter, Rudan, Salomaa, Satterfield, Schadt, Schipf, Scott, Sehmi, Shen, Soo Shin, Sigurdsson, Smith, Soranzo, Stančáková, Steinhagen-Thiessen, Streeten, Styrkarsdottir, Swart, Tan, Tarnopolsky, Thompson, Thomson, Thorsteinsdottir, Tikkanen, Tranah, Tuomilehto, van Schoor, Verma, Vollenweider, Völzke, Wactawski-Wende, Walker, Weedon, Welch, Wichmann, Widen, Williams, Wilson, Wright, Xie, Yu, Zhou, Chambers, Döring, van Duijn, Econs, Gudnason, Kooner, Psaty, Spector, Stefansson, Rivadeneira, Uitterlinden, Wareham, Ossowski, Waterworth, Loos, Karasik, Harris, Ohlsson, Kiel. Nature communications, 8(1). 2017.
T-Cell Mediation of Pregnancy Analgesia Affecting Chronic Pain in Mice. Rosen, Ham, Drouin, Boachie, Chabot-Dore, Austin, Diatchenko, Mogil. The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(41). 2017.
Car8 dorsal root ganglion expression and genetic regulation of analgesic responses are associated with a cis-eQTL in mice. Levitt, Zhuang, Kang, Erasso, Upadhyay, Ozdemir, Fu, Sarantopoulos, Smith, Maixner, Diatchenko, Martin, Wiltshire. Mammalian genome : official journal of the International Mammalian Genome Society, 28(9-10). 2017.
Epiregulin and EGFR interactions are involved in pain processing. Martin, Smith, Khoutorsky, Magnussen, Samoshkin, Sorge, Cho, Yosefpour, Sivaselvachandran, Tohyama, Cole, Khuong, Mir, Gibson, Wieskopf, Sotocinal, Austin, Meloto, Gitt, Gkogkas, Sonenberg, Greenspan, Fillingim, Ohrbach, Slade, Knott, Dubner, Nackley, Ribeiro-da-Silva, Neely, Maixner, Zaykin, Mogil, Diatchenko. The Journal of clinical investigation, 127(9). 2017.
Large meta-analysis of genome-wide association studies identifies five loci for lean body mass. Zillikens, Demissie, Hsu, Yerges-Armstrong, Chou, Stolk, Livshits, Broer, Johnson, Koller, Kutalik, Luan, Malkin, Ried, Smith, Thorleifsson, Vandenput, Hua Zhao, Zhang, Aghdassi, Åkesson, Amin, Baier, Barroso, Bennett, Bertram, Biffar, Bochud, Boehnke, Borecki, Buchman, Byberg, Campbell, Campos Obanda, Cauley, Cawthon, Cederberg, Chen, Cho, Jin Choi, Claussnitzer, Collins, Cummings, De Jager, Demuth, Dhonukshe-Rutten, Diatchenko, Eiriksdottir, Enneman, Erdos, Eriksson, Eriksson, Estrada, Evans, Feitosa, Fu, Garcia, Gieger, Girke, Glazer, Grallert, Grewal, Han, Hanson, Hayward, Hofman, Hoffman, Homuth, Hsueh, Hubal, Hubbard, Huffman, Husted, Illig, Ingelsson, Ittermann, Jansson, Jordan, Jula, Karlsson, Khaw, Kilpeläinen, Klopp, Kloth, Koistinen, Kraus, Kritchevsky, Kuulasmaa, Kuusisto, Laakso, Lahti, Lang, Langdahl, Launer, Lee, Lerch, Lewis, Lind, Lindgren, Liu, Liu, Liu, Ljunggren, Lorentzon, Luben, Maixner, McGuigan, Medina-Gomez, Meitinger, Melhus, Mellström, Melov, Michaëlsson, Mitchell, Morris, Mosekilde, Newman, Nielson, O'Connell, Oostra, Orwoll, Palotie, Parker, Peacock, Perola, Peters, Polasek, Prince, Räikkönen, Ralston, Ripatti, Robbins, Rotter, Rudan, Salomaa, Satterfield, Schadt, Schipf, Scott, Sehmi, Shen, Soo Shin, Sigurdsson, Smith, Soranzo, Stančáková, Steinhagen-Thiessen, Streeten, Styrkarsdottir, Swart, Tan, Tarnopolsky, Thompson, Thomson, Thorsteinsdottir, Tikkanen, Tranah, Tuomilehto, van Schoor, Verma, Vollenweider, Völzke, Wactawski-Wende, Walker, Weedon, Welch, Wichmann, Widen, Williams, Wilson, Wright, Xie, Yu, Zhou, Chambers, Döring, van Duijn, Econs, Gudnason, Kooner, Psaty, Spector, Stefansson, Rivadeneira, Uitterlinden, Wareham, Ossowski, Waterworth, Loos, Karasik, Harris, Ohlsson, Kiel. Nature communications, 8(1). 2017.
miR-183 cluster scales mechanical pain sensitivity by regulating basal and neuropathic pain genes. Peng, Li, Zhang, Bengtsson Gonzales, Parisien, Belfer, Usoskin, Abdo, Furlan, Häring, Lallemend, Harkany, Diatchenko, Hökfelt, Hjerling-Leffler, Ernfors. Science (New York, N.Y.), 356(6343). 2017.
N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. Liu, Zhou, Parisien, Dai, Diatchenko, Pan. Nucleic acids research, 45(10). 2017.
Effect of Human Genetic Variability on Gene Expression in Dorsal Root Ganglia and Association with Pain Phenotypes. Parisien, Khoury, Chabot-Doré, Sotocinal, Slade, Smith, Fillingim, Ohrbach, Greenspan, Maixner, Mogil, Belfer, Diatchenko. Cell reports, 19(9). 2017.
GWAS Identifies New Loci for Painful Temporomandibular Disorder: Hispanic Community Health Study/Study of Latinos. Sanders, Jain, Sofer, Kerr, Laurie, Shaffer, Marazita, Kaste, Slade, Fillingim, Ohrbach, Maixner, Kocher, Bernhardt, Teumer, Schwahn, Sipilä, Lähdesmäki, Männikkö, Pesonen, Järvelin, Rizzatti-Barbosa, Meloto, Ribeiro-Dasilva, Diatchenko, Serrano, Smith. Journal of dental research, 96(3). 2017.
Genetic variant rs3750625 in the 3'UTR of ADRA2A affects stress-dependent acute pain severity after trauma and alters a microRNA-34a regulatory site. Linnstaedt, Walker, Riker, Nyland, Hu, Rossi, Swor, Jones, Diatchenko, Bortsov, Peak, McLean. Pain, 158(2). 2017.
Genetic predictors of human chronic pain conditions. Zorina-Lichtenwalter, Meloto, Khoury, Diatchenko. Neuroscience, 338. 2016.
Painful Temporomandibular Disorder: Decade of Discovery from OPPERA Studies. Slade, Ohrbach, Greenspan, Fillingim, Bair, Sanders, Dubner, Diatchenko, Meloto, Smith, Maixner. Journal of dental research, 95(10). 2016.
Agonist-dependence of functional properties for common nonsynonymous variants of human transient receptor potential vanilloid 1. Wang, Joseph, Diatchenko, Ro, Chung. Pain, 157(7). 2016.
Identification of clusters of individuals relevant to temporomandibular disorders and other chronic pain conditions: the OPPERA study. Bair, Gaynor, Slade, Ohrbach, Fillingim, Greenspan, Dubner, Smith, Diatchenko, Maixner. Pain, 157(6). 2016.
Human Genetic Variability Contributes to Postoperative Morphine Consumption. De Gregori, Diatchenko, Ingelmo, Napolioni, Klepstad, Belfer, Molinaro, Garbin, Ranzani, Alberio, Normanno, Lovisari, Somaini, Govoni, Mura, Bugada, Niebel, Zorzetto, De Gregori, Molinaro, Fanelli, Allegri. The journal of pain : official journal of the American Pain Society, 17(5). 2016.
Modification of COMT-dependent pain sensitivity by psychological stress and sex. Meloto, Bortsov, Bair, Helgeson, Ostrom, Smith, Dubner, Slade, Fillingim, Greenspan, Ohrbach, Maixner, McLean, Diatchenko. Pain, 157(4). 2016.
N(6)-Methyladenosine Modification in a Long Noncoding RNA Hairpin Predisposes Its Conformation to Protein Binding. Zhou, Parisien, Dai, Liu, Diatchenko, Sachleben, Pan. Journal of molecular biology, 428(5 Pt A). 2016.
Structural and functional interactions between six-transmembrane μ-opioid receptors and β2-adrenoreceptors modulate opioid signaling. Samoshkin, Convertino, Viet, Wieskopf, Kambur, Marcovitz, Patel, Stone, Kalso, Mogil, Schmidt, Maixner, Dokholyan, Diatchenko. Scientific reports, 5. 2015.
Dual allosteric modulation of opioid antinociceptive potency by α2A-adrenoceptors. Chabot-Doré, Millecamps, Naso, Devost, Trieu, Piltonen, Diatchenko, Fairbanks, Wilcox, Hébert, Stone. Neuropharmacology, 99. 2015.
Differences in the Antinociceptive Effects and Binding Properties of Propranolol and Bupranolol Enantiomers. Martin, Piltonen, Gauthier, Convertino, Acland, Dokholyan, Mogil, Diatchenko, Maixner. The journal of pain : official journal of the American Pain Society, 16(12). 2015.
Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. Gao, Krokowski, Guan, Bederman, Majumder, Parisien, Diatchenko, Kabil, Willard, Banerjee, Wang, Bebek, Evans, Fox, Gerson, Hoppel, Liu, Arvan, Hatzoglou. eLife, 4. 2015.
Genome-wide association meta-analyses to identify common genetic variants associated with hallux valgus in Caucasian and African Americans. Hsu, Liu, Hannan, Maixner, Smith, Diatchenko, Golightly, Menz, Kraus, Doherty, Wilson, Jordan. Journal of medical genetics, 52(11). 2015.
Neuropathic pain phenotyping by international consensus (NeuroPPIC) for genetic studies: a NeuPSIG systematic review, Delphi survey, and expert panel recommendations. van Hecke, Kamerman, Attal, Baron, Bjornsdottir, Bennett, Bennett, Bouhassira, Diatchenko, Freeman, Freynhagen, Haanpää, Jensen, Raja, Rice, Seltzer, Thorgeirsson, Yarnitsky, Smith. Pain, 156(11). 2015.
μ-Opioid receptor 6-transmembrane isoform: A potential therapeutic target for new effective opioids. Convertino, Samoshkin, Gauthier, Gold, Maixner, Dokholyan, Diatchenko. Progress in neuro-psychopharmacology & biological psychiatry, 62. 2015.
Molecular genetic mechanisms of allelic specific regulation of murine Comt expression. Segall, Shabalina, Meloto, Wen, Cunningham, Tarantino, Wiltshire, Gauthier, Tohyama, Martin, Mogil, Diatchenko. Pain, 156(10). 2015.
COMT gene locus: new functional variants. Meloto, Segall, Smith, Parisien, Shabalina, Rizzatti-Barbosa, Gauthier, Tsao, Convertino, Piltonen, Slade, Fillingim, Greenspan, Ohrbach, Knott, Maixner, Zaykin, Dokholyan, Reenilä, Männistö, Diatchenko. Pain, 156(10). 2015.
COMT Diplotype Amplifies Effect of Stress on Risk of Temporomandibular Pain. Slade, Sanders, Ohrbach, Bair, Maixner, Greenspan, Fillingim, Smith, Diatchenko. Journal of dental research, 94(9). 2015.
OPRM1 receptor as new biomarker to help the prediction of post mastectomy pain and recurrence in breast cancer. De Gregori, Diatchenko, Belfer, Allegri. Minerva anestesiologica, 81(8). 2015.
The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors. Wieskopf, Mathur, Limapichat, Post, Al-Qazzaz, Sorge, Martin, Zaykin, Smith, Freitas, Austin, Dai, Zhang, Marcovitz, Tuttle, Slepian, Clarke, Drenan, Janes, Al Sharari, Segall, Aasvang, Lai, Bittner, Richards, Slade, Kehlet, Walker, Maskos, Changeux, Devor, Maixner, Diatchenko, Belfer, Dougherty, Su, Lummis, Imad Damaj, Lester, Patapoutian, Mogil. Science translational medicine, 7(287). 2015.
Subgrouping of low back pain patients for targeting treatments: evidence from genetic, psychological, and activity-related behavioral approaches. Huijnen, Rusu, Scholich, Meloto, Diatchenko. The Clinical journal of pain, 31(2). 2015.
Carbonic anhydrase-8 regulates inflammatory pain by inhibiting the ITPR1-cytosolic free calcium pathway. Zhuang, Keeler, Grant, Bianchi, Fu, Zhang, Erasso, Cui, Wiltshire, Li, Hao, Sarantopoulos, Candiotti, Wishnek, Smith, Maixner, Diatchenko, Martin, Levitt. PloS one, 10(3). 2015.
Differential Regulation of 6- and 7-Transmembrane Helix Variants of μ-Opioid Receptor in Response to Morphine Stimulation. Convertino, Samoshkin, Viet, Gauthier, Li Fraine, Sharif-Naeini, Schmidt, Maixner, Diatchenko, Dokholyan. PloS one, 10(11). 2015.
Epistasis between polymorphisms in COMT, ESR1, and GCH1 influences COMT enzyme activity and pain. Smith, Reenilä, Männistö, Slade, Maixner, Diatchenko, Nackley. Pain, 155(11). 2014.
Letting the gene out of the bottle: OPRM1 interactions. Belfer, Young, Diatchenko. Anesthesiology, 121(4). 2014.
Complex multilocus effects of catechol-O-methyltransferase haplotypes predict pain and pain interference 6 weeks after motor vehicle collision. Bortsov, Diatchenko, McLean. Neuromolecular medicine, 16(1). 2014.
Differential expression of the alternatively spliced OPRM1 isoform μ-opioid receptor-1K in HIV-infected individuals. Dever, Costin, Xu, El-Hage, Balinang, Samoshkin, O'Brien, McRae, Diatchenko, Knapp, Hauser. AIDS (London, England), 28(1). 2014.
Multivariable modeling of phenotypic risk factors for first-onset TMD: the OPPERA prospective cohort study. Bair, Ohrbach, Fillingim, Greenspan, Dubner, Diatchenko, Helgeson, Knott, Maixner, Slade. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Summary of findings from the OPPERA prospective cohort study of incidence of first-onset temporomandibular disorder: implications and future directions. Slade, Fillingim, Sanders, Bair, Greenspan, Ohrbach, Dubner, Diatchenko, Smith, Knott, Maixner. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Study protocol, sample characteristics, and loss to follow-up: the OPPERA prospective cohort study. Bair, Brownstein, Ohrbach, Greenspan, Dubner, Fillingim, Maixner, Smith, Diatchenko, Gonzalez, Gordon, Lim, Ribeiro-Dasilva, Dampier, Knott, Slade. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Signs and symptoms of first-onset TMD and sociodemographic predictors of its development: the OPPERA prospective cohort study. Slade, Bair, Greenspan, Dubner, Fillingim, Diatchenko, Maixner, Knott, Ohrbach. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Clinical orofacial characteristics associated with risk of first-onset TMD: the OPPERA prospective cohort study. Ohrbach, Bair, Fillingim, Gonzalez, Gordon, Lim, Ribeiro-Dasilva, Diatchenko, Dubner, Greenspan, Knott, Maixner, Smith, Slade. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Pain sensitivity and autonomic factors associated with development of TMD: the OPPERA prospective cohort study. Greenspan, Slade, Bair, Dubner, Fillingim, Ohrbach, Knott, Diatchenko, Liu, Maixner. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Psychological factors associated with development of TMD: the OPPERA prospective cohort study. Fillingim, Ohrbach, Greenspan, Knott, Diatchenko, Dubner, Bair, Baraian, Mack, Slade, Maixner. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Genetic variants associated with development of TMD and its intermediate phenotypes: the genetic architecture of TMD in the OPPERA prospective cohort study. Smith, Mir, Bair, Slade, Dubner, Fillingim, Greenspan, Ohrbach, Knott, Weir, Maixner, Diatchenko. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Facial pain with localized and widespread manifestations: separate pathways of vulnerability. Slade, Smith, Zaykin, Tchivileva, Gibson, Yuryev, Mazo, Bair, Fillingim, Ohrbach, Greenspan, Maixner, Diatchenko. Pain, 154(11). 2013.
Multisystem dysregulation in painful temporomandibular disorders. Chen, Nackley, Miller, Diatchenko, Maixner. The journal of pain : official journal of the American Pain Society, 14(9). 2013.
Pain modality- and sex-specific effects of COMT genetic functional variants. Belfer, Segall, Lariviere, Smith, Dai, Slade, Rashid, Mogil, Campbell, Edwards, Liu, Bair, Maixner, Diatchenko. Pain, 154(8). 2013.
Polymorphisms in the glucocorticoid receptor co-chaperone FKBP5 predict persistent musculoskeletal pain after traumatic stress exposure. Bortsov, Smith, Diatchenko, Soward, Ulirsch, Rossi, Swor, Hauda, Peak, Jones, Holbrook, Rathlev, Foley, Lee, Collette, Domeier, Hendry, McLean. Pain, 154(8). 2013.
Sleep apnea symptoms and risk of temporomandibular disorder: OPPERA cohort. Sanders, Essick, Fillingim, Knott, Ohrbach, Greenspan, Diatchenko, Maixner, Dubner, Bair, Miller, Slade. Journal of dental research, 92(7 Suppl). 2013.
The phenotypic and genetic signatures of common musculoskeletal pain conditions. Diatchenko, Fillingim, Smith, Maixner. Nature reviews. Rheumatology, 9(6). 2013.
Correlation of transcription of MALAT-1, a novel noncoding RNA, with deregulated expression of tumor suppressor p53 in small DNA tumor virus models. Jeffers, Duan, Ellies, Seaman, Burger-Calderon, Diatchenko, Webster-Cyriaque. Journal of cancer therapy, 4(3). 2013.
Relationship between temporomandibular disorders, widespread palpation tenderness, and multiple pain conditions: a case-control study. Chen, Slade, Lim, Miller, Maixner, Diatchenko. The journal of pain : official journal of the American Pain Society, 13(10). 2012.
Janus molecule I: dichotomous effects of COMT in neuropathic vs nociceptive pain modalities. Segall, Maixner, Belfer, Wiltshire, Seltzer, Diatchenko. CNS & neurological disorders drug targets, 11(3). 2012.
Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Tsao, Wieskopf, Rashid, Sorge, Redler, Segall, Mogil, Maixner, Dokholyan, Diatchenko. Molecular pain, 8. 2012.
Genetically determined P2X7 receptor pore formation regulates variability in chronic pain sensitivity. Sorge, Trang, Dorfman, Smith, Beggs, Ritchie, Austin, Zaykin, Vander Meulen, Costigan, Herbert, Yarkoni-Abitbul, Tichauer, Livneh, Gershon, Zheng, Tan, John, Slade, Jordan, Woolf, Peltz, Maixner, Diatchenko, Seltzer, Salter, Mogil. Nature medicine, 18(4). 2012.
Large candidate gene association study reveals genetic risk factors and therapeutic targets for fibromyalgia. Smith, Maixner, Fillingim, Slade, Gracely, Ambrose, Zaykin, Hyde, John, Tan, Maixner, Diatchenko. Arthritis and rheumatism, 64(2). 2012.
Excess risk of temporomandibular disorder associated with cigarette smoking in young adults. Sanders, Maixner, Nackley, Diatchenko, By, Miller, Slade. The journal of pain : official journal of the American Pain Society, 13(1). 2012.
Catechol-O-methyltransferase genotype predicts pain severity in hospitalized burn patients. Orrey, Bortsov, Hoskins, Shupp, Jones, Cicuto, Hwang, Jordan, Holmes, Haith, Roane, Diatchenko, Cairns, McLean. Journal of burn care & research : official publication of the American Burn Association, 33(4). 2012.
Construction of a global pain systems network highlights phospholipid signaling as a regulator of heat nociception. Neely, Rao, Costigan, Mair, Racz, Milinkeviciute, Meixner, Nayanala, Griffin, Belfer, Dai, Smith, Diatchenko, Marengo, Haubner, Novatchkova, Gibson, Maixner, Pospisilik, Hirsch, Whishaw, Zimmer, Gupta, Sasaki, Kanaho, Sasaki, Kress, Woolf, Penninger. PLoS genetics, 8(12). 2012.
Cytokine biomarkers and chronic pain: association of genes, transcription, and circulating proteins with temporomandibular disorders and widespread palpation tenderness. Slade, Conrad, Diatchenko, Rashid, Zhong, Smith, Rhodes, Medvedev, Makarov, Maixner, Nackley. Pain, 152(12). 2011.
Relax, you won't feel the pain. Wiltshire, Maixner, Diatchenko. Nature neuroscience, 14(12). 2011.
Elucidation of mu-Opioid Gene Structure: How Genetics Can Help Predict Responses to Opioids. Diatchenko, Robinson, Maixner. European journal of pain supplements, 5(2). 2011.
Structural basis for μ-opioid receptor binding and activation. Serohijos, Yin, Ding, Gauthier, Gibson, Maixner, Dokholyan, Diatchenko. Structure (London, England : 1993), 19(11). 2011.
Summary of findings from the OPPERA baseline case-control study: implications and future directions. Fillingim, Slade, Diatchenko, Dubner, Greenspan, Knott, Ohrbach, Maixner. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Study methods, recruitment, sociodemographic findings, and demographic representativeness in the OPPERA study. Slade, Bair, By, Mulkey, Baraian, Rothwell, Reynolds, Miller, Gonzalez, Gordon, Ribeiro-Dasilva, Lim, Greenspan, Dubner, Fillingim, Diatchenko, Maixner, Dampier, Knott, Ohrbach. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Orofacial pain prospective evaluation and risk assessment study--the OPPERA study. Maixner, Diatchenko, Dubner, Fillingim, Greenspan, Knott, Ohrbach, Weir, Slade. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Potential autonomic risk factors for chronic TMD: descriptive data and empirically identified domains from the OPPERA case-control study. Maixner, Greenspan, Dubner, Bair, Mulkey, Miller, Knott, Slade, Ohrbach, Diatchenko, Fillingim. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Potential genetic risk factors for chronic TMD: genetic associations from the OPPERA case control study. Smith, Maixner, Greenspan, Dubner, Fillingim, Ohrbach, Knott, Slade, Bair, Gibson, Zaykin, Weir, Maixner, Diatchenko. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Disruptive mRNA folding increases translational efficiency of catechol-O-methyltransferase variant. Tsao, Shabalina, Gauthier, Dokholyan, Diatchenko. Nucleic acids research, 39(14). 2011.
Catechol O-methyltransferase haplotype predicts immediate musculoskeletal neck pain and psychological symptoms after motor vehicle collision. McLean, Diatchenko, Lee, Swor, Domeier, Jones, Jones, Reed, Harris, Maixner, Clauw, Liberzon. The journal of pain : official journal of the American Pain Society, 12(1). 2011.
Structural mechanism of S-adenosyl methionine binding to catechol O-methyltransferase. Tsao, Diatchenko, Dokholyan. PloS one, 6(8). 2011.
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely, Hess, Costigan, Keene, Goulas, Langeslag, Griffin, Belfer, Dai, Smith, Diatchenko, Gupta, Xia, Amann, Kreitz, Heindl-Erdmann, Wolz, Ly, Arora, Sarangi, Dan, Novatchkova, Rosenzweig, Gibson, Truong, Schramek, Zoranovic, Cronin, Angjeli, Brune, Dietzl, Maixner, Meixner, Thomas, Pospisilik, Alenius, Kress, Subramaniam, Garrity, Bellen, Woolf, Penninger. Cell, 143(4). 2010.
Comt1 genotype and expression predicts anxiety and nociceptive sensitivity in inbred strains of mice. Segall, Nackley, Diatchenko, Lariviere, Lu, Marron, Grabowski-Boase, Walker, Slade, Gauthier, Bailey, Steffy, Maynard, Tarantino, Wiltshire. Genes, brain, and behavior, 9(8). 2010.
Multiple chronic pain states are associated with a common amino acid-changing allele in KCNS1. Costigan, Belfer, Griffin, Dai, Barrett, Coppola, Wu, Kiselycznyk, Poddar, Lu, Diatchenko, Smith, Cobos, Zaykin, Allchorne, Gershon, Livneh, Shen, Nikolajsen, Karppinen, Männikkö, Kelempisioti, Goldman, Maixner, Geschwind, Max, Seltzer, Woolf. Brain : a journal of neurology, 133(9). 2010.
A novel alternatively spliced isoform of the mu-opioid receptor: functional antagonism. Gris, Gauthier, Cheng, Gibson, Gris, Laur, Pierson, Wentworth, Nackley, Maixner, Diatchenko. Molecular pain, 6. 2010.
Effect of catechol-O-methyltransferase polymorphism on response to propranolol therapy in chronic musculoskeletal pain: a randomized, double-blind, placebo-controlled, crossover pilot study. Tchivileva, Lim, Smith, Slade, Diatchenko, McLean, Maixner. Pharmacogenetics and genomics, 20(4). 2010.
Pain perception is altered by a nucleotide polymorphism in SCN9A. Reimann, Cox, Belfer, Diatchenko, Zaykin, McHale, Drenth, Dai, Wheeler, Sanders, Wood, Wu, Karppinen, Nikolajsen, Männikkö, Max, Kiselycznyk, Poddar, Te Morsche, Smith, Gibson, Kelempisioti, Maixner, Gribble, Woods. Proceedings of the National Academy of Sciences of the United States of America, 107(11). 2010.
Assessing potential functionality of catechol-O-methyltransferase (COMT) polymorphisms associated with pain sensitivity and temporomandibular joint disorders. Nackley, Diatchenko. Methods in molecular biology (Clifton, N.J.), 617. 2010.
Molecular assays for characterization of alternatively spliced isoforms of the u opioid receptor (MOR). Gris, Cheng, Pierson, Maixner, Diatchenko. Methods in molecular biology (Clifton, N.J.), 617. 2010.
Signaling pathways mediating beta3-adrenergic receptor-induced production of interleukin-6 in adipocytes. Tchivileva, Tan, Gambarian, Nackley, Medvedev, Romanov, Flood, Maixner, Makarov, Diatchenko. Molecular immunology, 46(11-12). 2009.
Characterization of NF-kB-mediated inhibition of catechol-O-methyltransferase. Tchivileva, Nackley, Qian, Wentworth, Conrad, Diatchenko. Molecular pain, 5. 2009.
Expansion of the human mu-opioid receptor gene architecture: novel functional variants. Shabalina, Zaykin, Gris, Ogurtsov, Gauthier, Shibata, Tchivileva, Belfer, Mishra, Kiselycznyk, Wallace, Staud, Spiridonov, Max, Goldman, Fillingim, Maixner, Diatchenko. Human molecular genetics, 18(6). 2009.
Haplotype associations with quantitative traits in the presence of complex multilocus and heterogeneous effects. Shibata, Diatchenko, Zaykin. Genetic epidemiology, 33(1). 2009.
Low enzymatic activity haplotypes of the human catechol-O-methyltransferase gene: enrichment for marker SNPs. Nackley, Shabalina, Lambert, Conrad, Gibson, Spiridonov, Satterfield, Diatchenko. PloS one, 4(4). 2009.
Orthodontic Treatment, Genetic Factors and Risk of Temporomandibular Disorder. Slade, Diatchenko, Ohrbach, Maixner. Seminars in orthodontics, 14(2). 2008.
Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors. Romanov, Medvedev, Gambarian, Poltoratskaya, Moeser, Medvedeva, Gambarian, Diatchenko, Makarov. Nature methods, 5(3). 2008.
Genetic architecture of human pain perception. Diatchenko, Nackley, Tchivileva, Shabalina, Maixner. Trends in genetics : TIG, 23(12). 2007.
Influence of psychological factors on risk of temporomandibular disorders. Slade, Diatchenko, Bhalang, Sigurdsson, Fillingim, Belfer, Max, Goldman, Maixner. Journal of dental research, 86(11). 2007.
Responses to Drs. Kim and Dionne regarding comments on Diatchenko, et al. Catechol-O-methyltransferase gene polymorphisms are associated with multiple pain-evoking stimuli. Pain 2006; 125: 216-24. Diatchenko, Slade, Nackley, Maixner. Pain, 129(3). 2007.
Catechol-O-methyltransferase inhibition increases pain sensitivity through activation of both beta2- and beta3-adrenergic receptors. Nackley, Tan, Fecho, Flood, Diatchenko, Maixner. Pain, 128(3). 2007.
Beta2 adrenergic receptor activation stimulates pro-inflammatory cytokine production in macrophages via PKA- and NF-kappaB-independent mechanisms. Tan, Nackley, Satterfield, Maixner, Diatchenko, Flood. Cellular signalling, 19(2). 2007.
Human catechol-O-methyltransferase haplotypes modulate protein expression by altering mRNA secondary structure. Nackley, Shabalina, Tchivileva, Satterfield, Korchynskyi, Makarov, Maixner, Diatchenko. Science (New York, N.Y.), 314(5807). 2006.
Catechol-O-methyltransferase gene polymorphisms are associated with multiple pain-evoking stimuli. Diatchenko, Nackley, Slade, Bhalang, Belfer, Max, Goldman, Maixner. Pain, 125(3). 2006.
GTP cyclohydrolase and tetrahydrobiopterin regulate pain sensitivity and persistence. Tegeder, Costigan, Griffin, Abele, Belfer, Schmidt, Ehnert, Nejim, Marian, Scholz, Wu, Allchorne, Diatchenko, Binshtok, Goldman, Adolph, Sama, Atlas, Carlezon, Parsegian, Lötsch, Fillingim, Maixner, Geisslinger, Max, Woolf. Nature medicine, 12(11). 2006.
Idiopathic pain disorders--pathways of vulnerability. Diatchenko, Nackley, Slade, Fillingim, Maixner. Pain, 123(3). 2006.
Three major haplotypes of the beta2 adrenergic receptor define psychological profile, blood pressure, and the risk for development of a common musculoskeletal pain disorder. Diatchenko, Anderson, Slade, Fillingim, Shabalina, Higgins, Sama, Belfer, Goldman, Max, Weir, Maixner. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 141B(5). 2006.
Perspectives on the genetic basis of opioid-induced hyperalgesia. Nackley, Diatchenko, Maixner. Anesthesiology, 104(5). 2006.
Identification of novel mediators of NF-kappaB through genome-wide survey of monocyte adherence-induced genes. Diatchenko, Romanov, Malinina, Clarke, Tchivilev, Li, Makarov. Journal of leukocyte biology, 78(6). 2005.
Genetic basis for individual variations in pain perception and the development of a chronic pain condition. Diatchenko, Slade, Nackley, Bhalang, Sigurdsson, Belfer, Goldman, Xu, Shabalina, Shagin, Max, Makarov, Maixner. Human molecular genetics, 14(1). 2005.
Gene expression analysis of purified hematopoietic stem cells and committed progenitors. Terskikh, Miyamoto, Chang, Diatchenko, Weissman. Blood, 102(1). 2003.
Generation of full-length cDNA libraries enriched for differentially expressed genes. Zhumabayeva, Chang, McKinley, Diatchenko, Siebert. Methods in molecular biology (Clifton, N.J.), 221. 2003.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer, Rubin, Hong, Stapleton, Soares, Bonaldo, Casavant, Scheetz, Brownstein, Usdin, Toshiyuki, Carninci, Prange, Raha, Loquellano, Peters, Abramson, Mullahy, Bosak, McEwan, McKernan, Malek, Gunaratne, Richards, Worley, Hale, Garcia, Gay, Hulyk, Villalon, Muzny, Sodergren, Lu, Gibbs, Fahey, Helton, Ketteman, Madan, Rodrigues, Sanchez, Whiting, Madan, Young, Shevchenko, Bouffard, Blakesley, Touchman, Green, Dickson, Rodriguez, Grimwood, Schmutz, Myers, Butterfield, Krzywinski, Skalska, Smailus, Schnerch, Schein, Jones, Marra. Proceedings of the National Academy of Sciences of the United States of America, 99(26). 2002.
Subtractive cloning: new genes for studying inflammatory disorders. Rebrikov, Desai, Kogan, Thornton, Diatchenko. Annals of periodontology, 7(1). 2002.
Gene expression profiling in RAS oncogene-transformed cell lines and in solid tumors using subtractive suppression hybridization and cDNA arrays. Sers, Tchernitsa, Zuber, Diatchenko, Zhumabayeva, Desai, Htun, Hyder, Wiechen, Agoulnik, Scharff, Siebert, Schäfer. Advances in enzyme regulation, 42. 2002.
Caveolin-1 is down-regulated in human ovarian carcinoma and acts as a candidate tumor suppressor gene. Wiechen, Diatchenko, Agoulnik, Scharff, Schober, Arlt, Zhumabayeva, Siebert, Dietel, Schäfer, Sers. The American journal of pathology, 159(5). 2001.
Generation of full-length cDNA libraries enriched for differentially expressed genes for functional genomics. Zhumabayeva, Chang, McKinley, Diatchenko, Siebert. BioTechniques, 30(3). 2001.
Use of SMART-generated cDNA for gene expression studies in multiple human tumors. Zhumabayeva, Diatchenko, Chenchik, Siebert. BioTechniques, 30(1). 2001.
Structure and regulation of the mouse ing1 gene. Three alternative transcripts encode two phd finger proteins that have opposite effects on p53 function. Zeremski, Hill, Kwek, Grigorian, Gurova, Garkavtsev, Diatchenko, Koonin, Gudkov. The Journal of biological chemistry, 274(45). 1999.
Amplification of cDNA ends based on template-switching effect and step-out PCR. Matz, Shagin, Bogdanova, Britanova, Lukyanov, Diatchenko, Chenchik. Nucleic acids research, 27(6). 1999.
Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. Diatchenko, Lukyanov, Lau, Siebert. Methods in enzymology, 303. 1999.
PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Akopyants, Fradkov, Diatchenko, Hill, Siebert, Lukyanov, Sverdlov, Berg. Proceedings of the National Academy of Sciences of the United States of America, 95(22). 1998.
Stress-induced secretion of growth inhibitors: a novel tumor suppressor function of p53. Komarova, Diatchenko, Rokhlin, Hill, Wang, Krivokrysenko, Feinstein, Gudkov. Oncogene, 17(9). 1998.
Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments. Fradkov, Lukyanov, Matz, Diatchenko, Siebert, Lukyanov. Analytical biochemistry, 258(1). 1998.
Differential screening of a subtracted cDNA library: a method to search for genes preferentially expressed in multiple tissues. Jin, Cheng, Diatchenko, Siebert, Huang. BioTechniques, 23(6). 1997.
Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Lukyanov, Diatchenko, Chenchik, Nanisetti, Siebert, Usman, Matz, Lukyanov. Biochemical and biophysical research communications, 230(2). 1997.
Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate. Gurskaya, Diatchenko, Chenchik, Siebert, Khaspekov, Lukyanov, Vagner, Ermolaeva, Lukyanov, Sverdlov. Analytical biochemistry, 240(1). 1996.
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Diatchenko, Lau, Campbell, Chenchik, Moqadam, Huang, Lukyanov, Lukyanov, Gurskaya, Sverdlov, Siebert. Proceedings of the National Academy of Sciences of the United States of America, 93(12). 1996.
Peer-Reviewed Publications
News

News


Dr. Luda Diatchenko is covered in Health EuropaSeptember 5, 2019

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Dr. Luda Diatchenko becomes Jens Christian Skou honorary professor2019

Dr. Luda Diatchenko becomes the Jens Christian Skou honorary professor at Aarhus University, DK.

Dr. Luda Diatchenko wins American Pain Society, The Future of Pain Science Award2019

Dr. Luda Diatchenko wins the American Pain Society's "The Future of Pain Science Award" (in recognition of chairing scientific program committee).

Vivek Verma awarded travel award2019

Vivek Verma was awarded the 2019 ISAC Travel Award for attendance at CYTO 2019 - ISAC’s 34th International Congress, Vancouver, June 22-26, 2019.

Dr. Luda Diatchenko is interviewed by Pain Management from Future MedicineJune 5, 2018

Pain Management from Future Medicine interviews Dr. Luda Diatchenko in "The human pain genetics database: an interview with Luda Diatchenko"

Samar Khoury awarded 16th Kresimir Research Award for Outstanding Research TraineeMay 31, 2018

Samar Khoury was awarded the 16th Kresimir Research Award for Outstanding Research Trainee for her work in “Genome-wide association study and meta-analysis identify NPY and MPP6 as novel genes in sleep quality” by the Department of Anesthesia, McGill University, Montreal, Quebec, Canada

Dr. Luda Diatchenko appears on the CBCMarch 13, 2018

In a "Ms. Scientist" interview about women and science for the CBC, Dr. Luda Diatchenko appears.

Carolina Meloto awarded Catherine Bushnell Fellowship2018

Carolina Meloto was awarded the Catherine Bushnell Fellowship in Chronic Pain Research, The Louise and Alan Edwards Foundation, Montreal, Quebec, Canada.

Katerina Zorina-Lichtenwalter awarded travel grant2017

Katerina Lichtenwalter was awarded a travel grant of $500 from Graduate Research Enhancement and Travel (GREAT) to attend the Canadian Pain Society Meeting May 23-26, 2017.

Stefano Cattaneo awarded Department of Experimental Medicine travel grantJuly 28, 2016

Stefano Cattaneo was awarded a travel grant $1250 from the Department of Experimental Medicine, McGill University to attend the International Association for the Study of Pain (IASP) 16th World Congress on Pain, Yokohama, Japan, September 26-30, 2016.

Dr. Luda Diatchenko interviewed in "Genetics: An Incomplete Mosaic" Nature Outlook ArticleJuly 13, 2016

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Katerina Zorina-Lichtenwalter selected for oral presentationMay 24, 2016

Katerina Zorina-Lichtenwalter is selected to give an oral presentation on "Deciphering the role of melanocortin 1 receptor in pain through single nucleotide polymorphisms" during the Hot Topics Sessions at the annual Canadian Pain Society meeting in Vancouver, Canada.

Carol Meloto receives Kresimir Krnjevic Research AwardMay 18, 2016

Dr. Carol Meloto receives the 14th Kresimir Krnjevic Research Award for 2016.

Carol Meloto receives Catherine Bushnell Fellowship in Chronic Pain ResearchMay 17, 2016

Dr. Carol Meloto receives the Catherine Bushnell Fellowship in Chronic Pain Research for 2016.

Dr. Luda Diatchenko is interviewed for Relief NewsApril 28, 2016

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Katerina Zorina-Lichtenwalter receives QPRN travel grantApril 2016

Katerina Zorina-Lichtenwalter is awarded a travel grant of $1000 from the Quebec Pain Research Network (QPRN) to attend the Canadian Pain Society Meeting from May 24-27, 2016.

Stefano Cattaneo awarded Young Against Pain (YAP) best project award2016

Stefano Cattaneo was awarded the Young Against Pain (YAP) award for best project at the conference organized by the Study In Multidisciplinary Pain Research (SIMPAR 2016).

Carol Meloto wins best young researcher award2015

Dr. Carol Meloto wins Best Young Researcher at the 7th Study in Multidisciplinary Pain Research (SIMPAR) in Rome, Italy.

Publication of Pain Genetics: Basic to Translational ScienceJanuary 2014

Pain Genetics: Basic to Translational Science is a timely synthesis of the key areas of research informing our understanding of the genetic basis of pain. The book opens with foundational information on basic genetic mechanisms underlying pain perception and progresses recently discovered complex concepts facing the field. The coverage is wide-ranging and will serves as an excellent entry point into understating the genetics of pain as well as providing a single resource for established researchers looking for a better understanding of the diverse strands of research going on in the area. With contributors painstakingly selected to provide a broad range of perspectives and research, Pain Genetics will be a valuable resource for geneticists, neuroscientists, and biomedical professionals alike.

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Human pain genetics focus of new excellence research chairSeptember 30, 2013

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$10-million federal grant brings prominent pain researcher to McGillSeptember 26, 2013

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World-leading expert on pain genetics joining McGillSeptember 25, 2013

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Studies

Clinical Studies


Fibromyalgia Study

Low Back Pain Study

Quebec Low Back Pain Core Study (QLBP Core) and Quebec Low Back Pain Biobank (QLBP Biobank)

Opportunities page header image.

Opportunities


The Human Pain Genetics lab forms part of the Alan Edwards Centre for Research on Pain at McGill University, which includes researchers from the Faculties of Medicine, Dentistry and Science all centered on the study of pain. Being part of this prestigious centre not only facilitates an excellent environment for collaborations but also creates unique learning opportunities and offers exposure to a variety of approaches in pain research.

We encourage applicants with backgrounds from a variety of fields including: epidemiology, bioinformatics, statistics, statistical genetics, medicine, and nursing.

Any general questions about training or employment opportunities should be addressed to Francesca Montagna.

The currently available opportunities are:

Graduate Student in Bioinformatics

The Human Pain Genetics lab is seeking a highly motivated student to undertake a PhD program under the supervision of Dr. Luda Diatchenko. The candidate should have a strong background in computer science, bioinformatics or statistics, with keen interest in solving problems related to the genetics and/or genomics of pain.

The candidate should have demonstrable skills in UNIX, be proficient in at least one programming language (R, perl, python, C/C++, etc), statistics, data visualization, and possess knowledge of public bioinformatics datasets and tools. Furthermore, the candidate should possess well-rounded written and verbal communication skills in English, as well as a strong publication record in a relevant field.

The successful candidate will make use of the lab’s exclusive data sets as well as those in public databases to: 1) make specific predictions at the SNP, gene or pathway level about pathophysiology of pain, and/or 2) help explain or summarize existing findings in a bigger, integrated and unified concepts. Predictions can be experimentally verified using means of molecular biology, or at the whole organism level in an animal model of choice.

The Human Pain Genetics lab is part of the Alan Edwards Centre for Research on Pain, at McGill University. The center groups researchers from the Faculties of Medicine, Dentistry and Science. Researchers focus on the study of pain. Being part of this world-wide recognized Pain Centre not only facilitates an excellent environment for collaborations but also creates unique learning opportunities, and offers exposure to a variety of approaches in pain research. The centre’s approach is multi- disciplinary; bringing together people with expertise in bioinformatics, statistics, computational biology and chemistry, epidemiology, medicine and more.

As a requirement, the candidate should have completed a thesis-based Master’s degree or have a related background in computer science, human genetics or bioinformatics. The candidate should also meet the requirements of McGill University graduate admission criteria.

Candidates should send a cover letter, a current CV, a statement of interest, and two references’ email addresses, with “Graduate Student in Bioinformatics” as subject, to: Dr. Marc Parisien

Life in Montreal

Notre Dame of Montreal
Allez Up Climbing Gym
Montreal at Night
Another Montreal Picture

The great city of Montreal has been home to Dr. Diatchenko’s Human Pain Genetics lab since 2013. Montreal is a vibrant place that contrasts the cosmopolitan feeling of a big North American city with the local charm of the Québécois culture. We enjoy exploring the city during all seasons, and members of the lab often get together to enjoy urban markets, cultural events, and outdoor activities, such as hiking, biking, and climbing. Montreal is one of the top host cities for international events in North America, with festivals and activities happening all year such as the Montreal Jazz Festival, the Montreal Grand Prix and the International Festival of Circus Arts. The city also ranks highest in Canada for the the number of restaurants per capita, and we plan to try them all!

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Resources


Human Pain Genetics Database

The Human Pain Genes Database (HPGDB) is a comprehensive compilation of single nucleotide polymorphisms (SNPs) that have been reported to be associated with pain. Interpersonal variability in chronic pain etiology, acute pain sensitivity, and analgesic response has been suggestive of differences in genetic predisposition, a theory supported by the numerous genetic association studies published during the last twenty years. As common, generally low-effect markers of genetic variability, SNPs are instrumental in sampling this predisposition and in identification of risk-modifying genes. With an ever-growing number of studies, the HPGDB is a regularly-updated tabular summary of SNP-phenotype associations, populated via manually-curated literature review. It is intended to serve as a reference and to generate new hypotheses that may lead to a better understanding of underlying molecular mechanisms and consequently the development of personalized treatment.

Results are presented in the following six-column format: genetic locus (or loci where applicable), SNP rsID, allele or haplotype reported in association analysis, direction of effect, associated phenotype, and citation. Additional information is displayed upon hovering over each field, and fields provide links to the corresponding reference database in NCBI and PubMed. The data is downloadable in a variety of formats.

Human pain genetics database: a resource dedicated to human pain genetics research. Meloto CB, Benavides R, Lichtenwalter RN, Wen X, Tugarinov N, Zorina-Lichtenwalter K, Chabot-Doré AJ, Piltonen MH, Cattaneo S, Verma V, Klares R 3rd, Khoury S, Parisien M, Diatchenko L. Pain 159(4):749-763. 2019.

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DRG-eQTLs

Single nucleotide polymorphisms (SNPs) associated with the expression level of a gene or an exon are called expression quantitative trait loci (eQTLs). We have mapped eQTLs in human dorsal root ganglions (DRG), a tissue highly relevant for pain research. This resource provides processed eQTL data (SNP, gene, beta, p-value) and input files for matrix_eQTL (genotype, mRNA levels, covariates).

Effect of genetic variability on gene expression in dorsal root ganglia and association with pain phenotypes. Parisien M, Khoury S, Chabot-Doré AJ, Sotocinal SG, Slade GD, Smith SB, Fillingim RB, Ohrbach R, Greenspan JD, Maixner W, Mogil JS, Belfer I, Diatchenko L. Cell Reports 19(9):1940-1952. 2017.

Transcriptomes (CEL format; samples section) available in GEO.

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Improved mRMR

Improved mRMR is a re-implementation of the minimum redundancy maximum relevance (mRMR) feature selection algorithm with emphasis on greatly increased perfomance (1000x or greater on large data sets) and an improved user interface. There are no disadvantages to using this utility as opposed to the original release by Hanchuan Peng, but benefits include:

  • results identical to original mRMR implementation by Hanchuan Peng, excluding statistically inconsequential preservation of rank corresponce in the case of metric ties
  • incorporation of all improvements from the Fast-MRMR implementation by Sergio Ramírez
  • additional performance improvements, such as avoiding computing mutual information for zero-entropy attributes, and careful selection of and usage of data structures
  • output for each attribute includes its selection rank, entropy, mutual information with the class attribute, and mRMR score in an easily parsed format friendly to downstream manipulation
  • operates directly on original textual data, requiring no transformation into a one-time binary representation
  • robust data set parser fails gracefully with bad input and reports the location of the first error
  • modular support in the code for arbitrary discretization routines, with several examples already provided and implemented
  • support to output the result of parsing and discretization so that it can be verified and analyzed with external tools
  • supports stream-based processing, and can operate equally well reading a data set from standard input, in a pipeline, from a named pipe, or with process substitution
  • standard GNU getopt_long POSIX-compliant option processing, including full-featured -h/--help capability, informative error messages, graceful failure, and sensible defaults
  • high-quality C++14 compliant code base

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Orofacial Pain: Prospective Evaluation and Risk Assessment (OPPERA)

Temporomandibular disorders (TMD) are a family of heterogeneous, painful musculoskeletal disorders representing the most common chronic orofacial pain condition. In the 2002 National Health Interview Survey, 5% of US adults reported TMD-type pain, while an examination of a representative sample of females in New York City found that 10% had examiner-diagnosed TMD. In two follow-up studies of individuals diagnosed with TMD, TMD persisted for up to five years for 51-67% of these individuals. TMD is a substantial societal burden, affecting one’s entire life, estimated to result in 17.8 million lost work days per year for every 100 million working adults in the US. Further, treatment for a those with persistent pain and dysfunction represents about 85% of the cost of treating TMD. Since the molecular pathophysiology of TMD remains unknown, there is a critical need to identify the sequence of genetic, molecular, cellular, and systemic events that lead to the onset and persistence of TMD, including development of specific risk factors.

Genome-wide association reveals contribution of MRAS to painful temporomandibular disorder in males. Smith SB, Parisien M, Bair E, Belfer I, Chabot-Doré AJ, Gris P, Khoury S, Tansley S, Torosyan Y, Zaykin DV, Bernhardt O, de Oliveira Serrano P, Gracely RH, Jain D, Järvelin MR, Kaste LM, Kerr KF, Kocher T, Lähdesmäki R, Laniado N, Laurie CC, Laurie CA, Männikkö M, Meloto CB, Nackley AG, Nelson SC, Pesonen P, Ribeiro-Dasilva MC, Rizzatti-Barbosa CM, Sanders AE, Schwahn C, Sipilä K, Sofer T, Teumer A, Mogil JS, Fillingim RB, Greenspan JD, Ohrbach R, Slade GD, Maixner W, Diatchenko L.. Pain 160(3):579-591. 2019.

Genotypes and phenotypes available in dbGaP.

Transcriptomics of Mouse Pain Assays

Chronic pain is a debilitating and poorly treated condition the underlying molecular mechanisms of which are poorly understood. Nerve injury and inflammation cause alterations in gene expression in tissues associated with pain processing, supporting molecular and cellular mechanisms that maintain painful states. However, it is not known whether transcriptome changes can be used to reconstruct a molecular pathophysiology of pain. In the current study, we identify molecular pathways contributing to chronic pain states through the analysis of global changes in the transcriptome of dorsal root ganglia, spinal cord, brain, and blood in mouse assays of nerve injury–induced and inflammation-induced pain. Comparative analyses of differentially expressed genes identified substantial similarities between these two animal pain assays and human low-back pain. Furthermore, the extracellular matrix (ECM) organization has been found the most commonly regulated pathway across all tested tissues in these two animal assays. In summary, our comprehensive transcriptomics analysis identified ECM organization as a central molecular pathway in the development of chronic pain.

Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain. Parisien M, Samoshkin A, Tansley SN, Piltonen MH, Martin LJ, El-Hachem N, Dagostino C, Allegri M, Mogil JS, Khoutorsky A, Diatchenko L. Pain 160(4):932-944. 2019.

Transcriptomes (fastq format; samples section) available in GEO.

List of differentially expressed genes (xlsx format; supplementary files table) available in GEO.

Contact Us

Contact Us

Human Pain Genetics Lab
Alan Edwards Centre for Research on Pain
Genome Building, Room 2201, 740 Dr. Penfield Avenue
Montreal, Quebec, Canada H3A 0G1
T: 514-398-2878 | F: 514-398-8900